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UICC World Cancer Congress 2006

Bridging the Gap: Transforming Knowledge into Action

July 8-12, 2006, Washington, DC, USA

Tuesday, 11 July 2006 - 12:00 PM
167-26

Polymorphisms in the Il-10 Gene Are Correlated with Levels of Il-10 Protein in Ovarian Cancer(Oc)

Elena Ioana Braicu, PhD, Student1, Ionela Cristina Pirvulescu, PhD, Student2, Alexandru Mustea, MD3, Dominique Könsgen, MD3, and Jalid Sehouli, PhD4. (1) Clinic for Obstetrics and Gynecology, University of Medicine, str. 21 Decembrie 1989 Nr. 57, Cluj-Napoca, 400124, Romania, (2) Department of Obstretics and Gynaecology, Iuliu Hatieganu Medical University Cluj-Napoca, Romania, TOC Tumorbank Ovarian Cancer Charité, Str. 21 Decembrie 1989, Cluj-Napoca, 400124, Romania, (3) Department of Gynecology, Campus Virchow Clinic, Charité Universitätsmedizin, Berlin, Germany, Augustenburger Platz Nr 1, Berlin, 13353, Germany, (4) Department of Gynecology, Campus Virchow Clinic, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, Berlin, 13353, Germany

Objective: IL1 and IL10 are supposed to be critically involved in the carcinogenesis of malignant solid tumors. This study was performed to investigate the possible influence of the polymorphisms of the IL-1A, IL-1B and IL-10 gene on the expression of the codified cytokines in patients with ovarian cancer.

Methods: In our study we genotyped 62 patients with OC and 39 patients without any malignancies (CG) for polymorphisms of IL-1 gene (IL-1A –889T/C and IL-1B –511C/T) and IL-10 gene (IL-10 -1082G/A, -819C/T and -592C/A), using pyrosequencing. We established the concentration of IL-1 and IL-10 in serum and ascites from 33 OC patients and 39 CG, 62 OC patients and 18 CG respectively, using ELISA technique. The DNA and Serum samples were obtained from the Tumor bank Ovarian Cancer (TOC).

Results: IL-10 allelic frequencies are different associated with the expression within patients with OC and CG. The A/A, C/T and A/C genotypes for -1082, -819 and -592 respectively showed significant higher IL-10 levels in serum in OC patients than in CG. No associations between polymorphisms within the IL-1 genes cluster and the expression of IL-1A and IL-1B in serum and ascites in OC and CG were found.The presence of IL-1A –889 T polymorphism seems to influence IL-1B expression in ascites in OC. The difference between IL-10 allelic distribution and the associated IL-10 serum levels in OC and CG, might suggest that tumor cells are able to produce themselves or to induce a higher IL-10 production not depending on the IL-10 promoter polymorphism.



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