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UICC World Cancer Congress 2006
Bridging the Gap: Transforming Knowledge into Action
July 8-12, 2006, Washington, DC, USA
Methods: In our study we genotyped 62 patients with OC and 39 patients without any malignancies (CG) for polymorphisms of IL-1 gene (IL-1A –889T/C and IL-1B –511C/T) and IL-10 gene (IL-10 -1082G/A, -819C/T and -592C/A), using pyrosequencing. We established the concentration of IL-1 and IL-10 in serum and ascites from 33 OC patients and 39 CG, 62 OC patients and 18 CG respectively, using ELISA technique. The DNA and Serum samples were obtained from the Tumor bank Ovarian Cancer (TOC).
Results: IL-10 allelic frequencies are different associated with the expression within patients with OC and CG. The A/A, C/T and A/C genotypes for -1082, -819 and -592 respectively showed significant higher IL-10 levels in serum in OC patients than in CG. No associations between polymorphisms within the IL-1 genes cluster and the expression of IL-1A and IL-1B in serum and ascites in OC and CG were found.The presence of IL-1A –889 T polymorphism seems to influence IL-1B expression in ascites in OC. The difference between IL-10 allelic distribution and the associated IL-10 serum levels in OC and CG, might suggest that tumor cells are able to produce themselves or to induce a higher IL-10 production not depending on the IL-10 promoter polymorphism.