UICC World Cancer Congress 2006

Objective: Hemofiltration (HF) is considered to be one of the most hemodynamically tolerant and efficient method in intensive care of sepsis no matter if the renal function is normal or disturbed. Proteins eliminated by HF have not been studied yet quantitatively and qualitatively to the full extent.

Methods: We have used proteomic methods in order to identify proteins eliminated into ultrafiltrate (UF). Ten studied cancer patients had postoperative sepsis complications on different days after surgery. UFs of this patient group were collected after HF procedure. Protein fractions were isolated from concentrated UFs by ion-exchange chromatography and proteins with molecular weight lower than 10 kDa were cut off. Then electrophoresis and MALDI-TOF masspectrometry were performed. In addition we measured amount of each identified protein in isolated protein mix by electrophoregramm scanning.

Results: Total amount of eliminated proteins was 552±45 mg per procedure of HF in average. Among identified proteins we detected following proteins: 14 kD (19.3% of total protein amount) corresponded to beta2-microglobulin; 25 kD (7.3%) – MHC I; 30 kD (9.7% ) – immunoglobulin k light chain variable region; 68 kD (20.6%) – serum albumin; 77kD (6.4%) – transferrin. Quantitative evaluation of removed proteins may be a useful tool for prognosis of these molecules loss while more long-term and subsequent detoxication procedures are performed. Development of methods for monitoring and compensation of eliminated essential proteins during HF should include such quantitative assessment.