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UICC World Cancer Congress 2006
Bridging the Gap: Transforming Knowledge into Action
July 8-12, 2006, Washington, DC, USA
Methods:Swiss albino mice administered with dietary p-dimethylaminoazobenzine(p-DAB) for five weeks were assessed for elemental profile and oxidant-antioxidant status. Energy-dispersive-X-ray-fluorescence(EDXRF) spectrometer was employed for the assessment of trace elements in lyophilized powdered liver samples. Assessment of intracellular oxidative stress was done microscopically and by luminescence spectrometer using dihydrorhodamine 123 in whole blood. Activity of antioxidant enzymes, superoxide dismutase(SOD), and catalase(CAT) were determined spectrophotometrically.
Results:Remarkable increase (>3-10 folds) in iron level noted in a time-dependant manner in liver and also its sub-cellular organelles of the p-DAB administered animals show possible association of iron overload with carcinogenic progression. Similar trend was observed for nickel only in the whole liver (~6 fold). Results show depletion in selenium level in both nucleus and mitochondria and depletion of Cu only in mitochondria. The observed enhancement of ROS production complies with the noted alterations in SOD and CAT. Data suggest intricate balance of specific elements involved in tuning of the molecular network of metabolic pathways.