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The 13th World Conference on Tobacco OR Health

Building capacity for a tobacco-free world

July 12-15, 2006, Washington, DC, USA

Thursday, July 13, 2006 - 12:00 PM

Surfactant Apo-Protein a (Sp-a) and Plasma Nicotine and Cotinine Levels in– Smoker and Nonsmoker Chronic Bronchitics

Digambar Behera, MD, D. Venkateswarlu, and Sidharatha Majumdar. Pulmonary Medicine, Postgraduate Institute of Medical Education & Research, Sector-12, Chandigarh, India

 Objective: To study the role of surfactant apo-protein A and the plasma nicotine and cotinine levels in smoker and non smoker Chronic bronchitic subjects. 

Methods: The SP- A level in the serum was estimated by ELISA immunoassay method.  Nicotine and cotinine were estimated by HPLC. Results were expressed in mean±SD and comparison was made by using student ‘t' test.


Thirty patients of chronic bronchitis who satisfied the selection criteria were taken up for the study (9 non-smokers ,21 smokers, age range 30-80 yrs; mean + SD 55.22+15.21).

The amount of surfactant recovered (table) from smoker bronchitics was significantly lower from that of non-smoker group  (p<0.001).The nicotine  and cotinine  levels were significantly higher in smoker bronchitics  compared to non-smoker group (p<0.001).



SP-A (mg/ml)

Nicotine (ng/ml)

Cotinine (ng/ml)

Smoker (n=21)




Nonsmoker (n=9)




P                                  <0.001                         <0.001                        <0.001 Conclusion: This study concludes that the surfactant Protein-A was low and nicotine and continine levels were significantly high in patients with smoker bronchitis in comparison to non-smoker bronchitis. The study may suggests that the nicotine and cotinine could be used as markers of smoking and the reduced levels of surfactant Protein-A could have a role in the pathogenesis of chronic bronchitis and could be a marker for early detection.

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